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Image Search Results
Journal: PLoS ONE
Article Title: Silencing of Doublecortin-Like (DCL) Results in Decreased Mitochondrial Activity and Delayed Neuroblastoma Tumor Growth
doi: 10.1371/journal.pone.0075752
Figure Lengend Snippet: Fold change in cytochrome c oxidase activity (A) and ATP synthesis (B) in shDCL-2 and shDCL-3 Dox-inducible NB cells treated for 72 hours with doxycycline (Dox) or vehicle (Veh). (C) DCL and α-tubulin expression in Dox-inducible NB cells treated with either Dox or Veh for 72 hours and subsequently transfected with DCL or empty plasmids. Cells were lysed for western blotting analysis 48 hours after transfection. (D) DCL expression normalized to α-tubulin. DCL expression is recovered in shDCL-2 and shDCL-3 cells after transfection with DCL plasmid (C-D). Fold change of cytochrome c oxidase activity (E) and ATP synthesis (F) in shDCL-2 and shDCL-3 cells treated with either Dox or Veh (72 hours) and then transfected with DCL or empty plasmid (48 hours). Fold changes were determined by normalizing to the negative control (NC) Dox-inducible NB cell line treated with Dox or Veh respectively. Cytochrome c oxidase activity was investigated in isolated mitochondria. Error bars, s.e.m. *, P <0.05; **, P <0.01; ***, P <0.001.
Article Snippet: Furthermore, results were normalized for membrane integrity of the outer mitochondrial membrane (
Techniques: Activity Assay, Expressing, Transfection, Western Blot, Plasmid Preparation, Negative Control, Isolation
Journal: PLoS ONE
Article Title: Silencing of Doublecortin-Like (DCL) Results in Decreased Mitochondrial Activity and Delayed Neuroblastoma Tumor Growth
doi: 10.1371/journal.pone.0075752
Figure Lengend Snippet: Cytochrome c oxidase activity (A) and ATP synthesis (B) in COS-1 cells transfected with empty vector, with DCL full-length or with one of the different DCL fragments subcloned into pDsRed2-N1 vector is shown. The structure of DCL full-length and the different DCL mutants is represented, indicating the DCX-domains and S/P-rich domain (SP). Error bars, s.e.m. *, P <0.05.
Article Snippet: Furthermore, results were normalized for membrane integrity of the outer mitochondrial membrane (
Techniques: Activity Assay, Transfection, Plasmid Preparation